EFFECTS OF MACRO ELEMENTS AND MEDIUM REPLENISHMENT ON CELL SUSPENSION CULTURE OF PANAX GINSENG HA ET GRUSHV. IN VITRO

Nguyễn Văn Kết, Trương Thị Lan Anh

Abstract


It has been demonstrated that the difference of macro-elements concentrations effect various on callus culture in vitro of Panax vietnamensis. The callus growths were the greatest in the medium supplemented with from 0.5 to 1.0- strength KNO3 and CaCl2, and 1.0-strength NH4H2PO4 and MgSO4. Moreover, the strategy of medium replenishment was helpful for cell suspension growth. And the maximum cell suspension growth was achieved with feeding of the 1/4MS at the end of 3rd.

Keywords


Bioreactor; Macro element; Medium replenishment; Panax vietnamensis Ha et Grushv

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DOI: http://dx.doi.org/10.37569/DalatUniversity.6.4.85(2016)

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