CLONING AND RESEARCHING METHYLATED LEVELS OF ICR AT Igf2/H19 LOCUS ON MICE (Mus musculus)

Abstract

Epigenetic is a phenomenon in the process of genomic imprinting called parentof-origin-specific is present allele depending on the allele origin. The molecular mechanisms of variables epigenetic: were imprinted by DNA methylation, histone acetylated proteins or chromosomal reorganisation. The expression of Igf2/H19 locus directly are affected by the methylated ICR (Imprinting control region), this area located on the imprinted locus Igf2/H19 has been cloned successfully, and their different levels of methylation depends on the allele derived from the father, mother and specific tissues. It was shown that the methylation regulates the expression of this locus directly.