A METHOD OF ISOLATION OF TANSHINONE IIA FROM SALVIA MILTIORRHIZA FOR WORKING REFERENCE STANDARD
Keywords:Tanshinone IIA, Salvia miltiorrhiza.
AbstractTanshinone IIA was isolated from the roots of Salvia miltiorrhiza for working reference standard. The method of isolation includes the extraction of the lipophillic components from the roots powder by n-hexane, the isolation of tanshinone IIA by silica gel column chromatography and purification of the compound by re-crystallisation. The compound was identified by its IR, NMR and Msspectra. The purity of the isolated compound were determined by HPLC to be more than 95%.
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