EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
DOI:
https://doi.org/10.37569/DalatUniversity.8.3.438(2018)Keywords:
Callus, Begonia bataiensis, Growth stimulant, In vitro.Abstract
Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate of clean samples was 81.7%. Leaves and stems of the transplanted plants in vitro were cultured on a half dish of MS media supplemented with 30g/l sucrose, 7.5g/l agar and plant growth-regulators: 2.4-Dichlorophenoxyacetic acid (2.4D) and thidiazuron (TDZ). Various concentration of TDZ (0; 0.1; 0.3; 0.5; 1 mg/l) were used. After 21 days, results were obtained for 96% of the callus on the medium supplemented with 0.3 mg/l TDZ. The callus had a fresh weight of 2,642 mg/sample and a dry weight of 271 mg/sample. The calluses were transferred onto a medium for forming shoots and roots to complete the plant in vitro.
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References
Frodin, D. G. (2004). History and concepts of big plant genera. Taxon, 53(3), 753-776.
Guse, W. E., Kumar, M., & Larsen, F. E. (2010). Propagation of plants from specialized structures. Retrieved from http://cru.cahe.wsu.edu/CEPublications/pnw164/PNW164.pdf.
Guse, W. E., & Larsen, F. E. (2001). Propagating herbaceous plants from cuttings. Retrieved from http://lewis-mg-mrc.org/yahoo_site_admin/assets/docs/pnw0151.167165125.pdf.
Hughes, M. (2008). An annotated checklist of Southeast Asian Begonia. Edinburgh, UK: Royal Botanic Garden Edinburgh.
Luong, T. H. (2013). Conservation and promotion of the value of the Kiengiang biosphere. Retrieved from http://biendoikhihau.longan.gov.vn/conservation-and-promotion-of-the-value-of-the-kien-giang-biosphere-p1021c1257.
Nguyen, H. Q., & Ku, M. S. (2010). Begonia vietnamensis, an attractive new species with peltate leaves from Vietnam. Begonian, 77, 18-21.
Peng, C. I., Lin, C. W., Yang, A. H., Kono, Y., & Nguyen, H. Q. (2015). Six new species of Begonia (Begoniaceae) from limestone areas in Northern Vietnam. Botanical Studies, 56(1), 1-23.
The Red List. (2018). Begonia bataiensis. Retrieved from http://www.iucnredlist.org/details/89663596/0.
Viện Sinh thái học miền Nam. (2009). Giới thiệu núi đá vôi Kiên Giang. Hà Nội, Việt Nam: NXB. Nông nghiệp.
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