INHIBITION OF CALCIUM OXALATE CRYSTALLIZATION CAUSING KIDNEY STONES IN VITRO BY AN EXTRACT OF Bluméa balsamifera

Authors

  • Nguyen Pham Tuan An Giang Biotechnology Center, Viet Nam,
  • Bang Hong Lam An Giang University, Viet Nam,
  • Nguyen Pham Tu An Giang Biotechnology Center, Viet Nam,
  • Le Thao Nguyen Gachon University, Korea, Republic of,
  • Tran Duc Tai Gachon University, Korea, Republic of,

DOI:

https://doi.org/10.37569/DalatUniversity.11.1.691(2021)

Keywords:

Aggregation, Bluméa balsamifera, Calcium oxalate, Growth, Nucleation.

Abstract

The inhibitory effect of Bluméa balsamifera extract on the nucleation, growth, and aggregation phases of calcium oxalate formation has been studied. Plant samples were extracted by the maceration method with 80.00% ethanol. The results showed that the moisture content of Bluméa balsamifera extract was 82.83% and the yield was 5.79%. Bluméa balsamifera extract contains bioactive compounds including alkaloids, flavonoids, saponins, terpenoids, tannins, and phenol. Bluméa balsamifera extract has the ability to inhibit the nucleation of calcium oxalate with an IC50 value of 4.25 mg/ml. Bluméa balsamifera extract has a significant inhibitory effect on the growth of calcium oxalate crystals with an IC50 value of 2.99 mg/ml. Finally, Bluméa balsamifera extract is capable of inhibiting the aggregation of calcium oxalate with an IC50 value of 2.56 mg/ml.

References

Agarwal, K., & Varma, R. (2015). In-vitro calcium oxalate crystallization inhibition by Achyranthes aspera L. and Bryophyllum pinnatum Lam. British Journal of Pharmaceutical Research, 5(2), 146-152.

Atmani, F., & Khan, S. R. (2000). Effects of an extract from Herniaria hirsuta on calcium oxalate crystallization in vitro. BJU International, 85(6), 621-625.

Atodariya, U., Barad, R., Upadhyay, S., & Upadhyay, U. (2013). Anti-Urolithiatic activity of Dolichos biflorus seeds. Journal of Pharmacognosy and Phytochemistry, 2(2), 209-213.

Bandar, H., Hijazi, A., Rammal, H., Hachem, A., Saad, Z., & Badran, B. (2013). Techniques for the extraction of bioactive compounds from Lebanese Urtica dioica. American Journal of Phytomedicine and Clinical Therapeutics, 6, 507-513.

Charlimagne, M. M., & Rizalinda, L. D. L. (2017). Effect of Blumea balsamifera extract on the phase and morphology of calcium oxalate crystals. Asian Journal of Urology, 4(4), 201-207.

Chaudhary, A., Singla, S. K., & Tandon, C. (2010). In vitro evaluation of Terminalia arjuna on calcium phosphate and calcium oxalate crystallization. Indian Journal of Pharmaceutical Sciences, 72(3), 340-345.

de Cógáin, M. R., Linnes, M. P., Lee, H. J., Krambeck, A. E., de Mendonça Uchôa, J. C., Kim, S. H., & Lieske, J. C. (2015). Aqueous extract of Costus arabicus inhibits calcium oxalate crystal growth and adhesion to renal epithelial cells. Urolithiasis, 43(2), 119-124.

Gupta, M., Bhayana, S., & Sikka, S. K. (2011). Role of urinary inhibitors and promoters in calcium oxalate crystallisation. International Journal of Research in Pharmacy and Chemistry, 1(4), 793-798.

Nirmaladevi, R., Kavitha, D., & Padma, P. R. (2012). Evaluation of antilithiatic potential of Hibiscus rosa-sinensis Linn, in vitro. Journal of Pharmacy Research, 5(8), 4353-4356.

Pareta, S. K., Prata, K. C., & Ranjit, K. H. (2011). In-vitro calcium oxalate crystallization inhibition by Achyranthes indica Linn. Hydroalcoholic extract: An approach to antilithiasis. International Journal of Pharma and Bio Sciences, 2(1), 432-437.

Phatak, R. S., & Hendre, A. S. (2015). In-vitro antiurolithiatic activity of Kalanchoe pinnata extract. International Journal of Pharmacognosy and Phytochemical Research, 7(2), 275-279.

Saha, S., & Verma, R. J. (2013). Inhibition of calcium oxalate crystallisation in vitro by an extract of Bergenia ciliata. Arab Journal of Urology, 11(2), 187-192.

Saranya, R., & Geetha, N. (2014). Inhibition of calcium oxalate (CaOx) crystallization in vitro by the extract of beet root (Beta vulgaris L.). International Journal of Pharmacy and Pharmaceutical Sciences, 6(2), 361-365.

Trần, Đ. T. (2016). Ảnh hưởng của dịch trích lá và thân cây Bùm sụm (Carmona microphylla L.) lên sự ức chế hình thành tinh thể Calcium oxalate gây bệnh sỏi thận trong điều kiện in vitro (Bachelor’s thesis, Can Tho University, Vietnam).

Vyawahare, J. N., Shelke, P. A., Aragade, P. D., & Baheti, D. G. (2014). Inhibition of calcium oxalate crystallization in vitro by extract of Momordica charantia Linn. International Journal of Pharmaceutical and Chemical Sciences, 3(2), 448-452.

Yadav, M., Chatterji, S., Gupta, S. K., & Watal, G. (2014). Preliminary phytochemical screening of six medicinal plants used in traditional medicine. International Journal of Pharmacy and Pharmaceutical Sciences, 6(5), 539-542.

Downloads

Published

05-02-2021

Volume and Issues

Section

Natural Sciences and Technology

How to Cite

Tuan, N. P., Lam, B. H., Tu, N. P., Nguyen, L. T., & Tai, T. D. (2021). INHIBITION OF CALCIUM OXALATE CRYSTALLIZATION CAUSING KIDNEY STONES IN VITRO BY AN EXTRACT OF Bluméa balsamifera. Dalat University Journal of Science, 11(1), 3-13. https://doi.org/10.37569/DalatUniversity.11.1.691(2021)