EFFECTS OF MACRO ELEMENTS AND MEDIUM REPLENISHMENT ON CELL SUSPENSION CULTURE OF PANAX GINSENG HA ET GRUSHV. IN VITRO

Authors

  • Nguyễn Văn Kết The Faculty of Agriculture and Forestry, Dalat University, Viet Nam
  • Trương Thị Lan Anh The Faculty of Agriculture and Forestry, Dalat University, Viet Nam

DOI:

https://doi.org/10.37569/DalatUniversity.6.4.85(2016)

Keywords:

Bioreactor, Macro element, Medium replenishment, Panax vietnamensis Ha et Grushv

Abstract

It has been demonstrated that the difference of macro-elements concentrations effect various on callus culture in vitro of Panax vietnamensis. The callus growths were the greatest in the medium supplemented with from 0.5 to 1.0- strength KNO3 and CaCl2, and 1.0-strength NH4H2PO4 and MgSO4. Moreover, the strategy of medium replenishment was helpful for cell suspension growth. And the maximum cell suspension growth was achieved with feeding of the 1/4MS at the end of 3rd.

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References

Đỗ, H. B., Đặng, Q. C., Bùi, X. C., Nguyễn, T. D., Đỗ, T. Đ., Phạm, V. H., Vũ, N. L., Phạm, D. M., Phạm, K. M., Đoàn, T. T., Nguyễn, T., & Trần, T. (2003). Cây thuốc và động vật làm thuốc ở Việt Nam (Tập II). Hà Nội, Việt Nam: NXB Khoa học và Kỹ thuật Hà Nội.

Furuya T., Yoshikawa T., Orihara Y., & Oda H. (1984). Studies of the culture onditions for Panax ginseng cells in jar fermentors. Journal of Natural Products, 47(1), 70-75.

Iranbakhsh, A. R., Oshagi, M. A., & Ebadi, M. (2007). Growth and production optimization of Tropane alkaloid in Datura stramonium cell suspension culture. Pakistan Journal of biological Sciences, 10(8), 1236-1242.

Jeong, C. S., Murthy, H. N., Hahn, E. J., & Paek K. Y. (2008). Improved production of ginsenosides in suspension cultures of ginseng by medium replenishment strategy. Journal of bioscience and bioengineering, 105(3), 288-291.

Liu, S., & Zhong, J. J. (1998). Phosphate effect on production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax notoginseng. Process Biochemistry, 33, 69-74.

Neto, F. A. G., Melanson, S. J., Sakata, K., & DiCosmo, F. (1993). Improved growth and taxol yield in developing callus of Taxus cuspidata by medium composition modification. Biological Technology, 11, 731-734.

Nguyễn, Đ. L., & Lê, T. T. T. (2002). Công nghệ tế bào. Thành phố Hồ Chí Minh, Việt Nam: NXB Đại học Quốc gia Thành phố Hồ Chí Minh.

Panda, A. K., Mishra, S., & Bisaria, V. S. (1992). Alkaloid production by plant cell suspension cultures of Holarrhena antidysenterica: Effect of major nutrients. Biotechnol Bioeng, 39, 1043-1051.

Srinivasan, V., & Ryu, D. D. Y. (1993). Improvement of shikonin productivity in Lithospernum erythrorhizon cell cultures by alternating carbon and nitrogen feeding strategy. Biotechnology and Bioengineering, 42, 793-799.

Thanh, N. T. (2005). Factor affecting cell growth and ginsenoside production in Panax ginseng C. A. Myer (Thesis for Degree of Doctor of Philosophy). Korea: Chungbuk National University.

Vinterhalter, D., & Vinterhalter, B. (1992). Effect of inorganic nutrition on the formation of lateral roots in Dracaena fragrans Ker-Gawl cultured in vitro. Plant Cell, Tissue and Organ Culture, 28, 267-274.

Wang, H. Q., Yu, J. T., & Zhong, J. J. (1999). Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy. Process Biochemistry, 35, 479-483.

Wu, C. H., Murthy, H. N., Hahn, E. J., & Paek, K. Y. (2007). Improved production of caffeic acid derivatives in suspension cultures of Echinacea purpurea by medium replenishment strategy. Archives of Pharmacal Research, 30(8), 945-949.

Yu, K. W., Gao, W. Y., Hahn, E. J., & Paek, K. Y. (2001). Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer). The Journal of Plant Biology, 44, 179-184.

Yu, K. W., Gao, W. Y., Son, S. H., & Paek, K. Y. (2000a). Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer). In Vitro Cellular & Developmental Biology, 36, 424-428.

Yu, K. W., Hahn, E. J., & Paek, K. Y. (2000b). Production of adventitious ginseng roots using bioreactors. Korean Journal of Plant Tissue Culture, 27, 309-315.

Zhang, Y. H., Zhong, J. J., & Yu, J. T. (1995). Effect of osmotic pressure on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnology Letters, 17(12), 1347-1350.

Zhang, Y. H., Zhong, J. J., & Yu, J. T. (1996). Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: Manipulation of medium sucrose. Journal Biotechnology, 51, 49-56.

Published

04-01-2017

Volume and Issues

Section

Natural Sciences and Technology

How to Cite

Kết, N. V., & Anh, T. T. L. (2017). EFFECTS OF MACRO ELEMENTS AND MEDIUM REPLENISHMENT ON CELL SUSPENSION CULTURE OF PANAX GINSENG HA ET GRUSHV. IN VITRO. Dalat University Journal of Science, 6(4), 419-430. https://doi.org/10.37569/DalatUniversity.6.4.85(2016)