POPULATION GENETIC DIVERSITY OF CAMELLIA DILINHENSIS ON THE DI LINH PLATEAU OF VIETNAM REVEALED BY ISSR AND SCOT MARKERS
DOI:
https://doi.org/10.37569/DalatUniversity.12.3.954(2022)Keywords:
Camellia dilinhensis, Genetic diversity, ISSR, Population, ScoT, Vietnam.Abstract
Genetic diversity of naturally distributed Camellia dilinhensis populations on the Di Linh plateau of Vietnam was assessed by the inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) techniques separately and then by combining data from both techniques for satisfactory results. The genetic diversity parameters and genetic distances among individuals found with the ISSR technique (HeI = 0.1420, II = 0.2092, PPBI = 35.22%, GSCsI = 0.765–0.988, and AGSCI = 0.915) are lower than those found with the SCoT technique (HeS = 0.2100, IS = 0.381, PPBS = 52.27%, GSCsS = 0.644–0.985, and AGSCS = 0.866). Based on the combined data from both techniques, the level of genetic diversity of the investigated population is PPB = 43.77%, He = 0.1720, I = 0.2582, and the genetic similarities among individuals are GSCs = 0.764–0.973 with an average of AGSC = 0.894. The SCoT technique differentiated between individuals better and reflected a higher level of genetic diversity in the population than the ISSR technique, but the ISSR technique revealed more loci in Camellia dilinhensis plants than did the SCoT technique.
References
Ahmed, M. A. (2005). PCR techniques. Department of Genetics, Zagazig University.
Amiryousefi, A., Hyvönen, J., & Poczai, P. (2018). iMEC: Online marker efficiency calculator. Applications in Plant Sciences, 6(6), e01159. https://doi.org/10.1002/aps3.1159
Beech, E., Barstow, M., & Rivers, M. (2017). The Red List of Theaceae. Botanic Gardens Conservation International.
Carling, M. D., & Brumfield, R. T. (2007). Gene sampling strategies for multi-locus population estimates of genetic diversity (θ). PLoS ONE, 2(1), e160. https://doi.org/10.1371/journal.pone.0000160
Chesnokov, Y. V., & Artemyeva, A. M. (2015). Evaluation of the measure of polymorphism information of genetic diversity. Agricultural Biology, 50(5), 571-578. https://doi.org/10.15389/agrobiology.2015.5.571eng
Collard, B. C. Y., & Mackill, D. J. (2009). Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27(1), 86-93. https://doi.org/10.1007/s11105-008-0060-5
Dang, Q. B., Nguyen, T. P. T., Nguyen, V. P., Ninh, T. T., Hoang, H. H., Vu, T. H. H., Doan, T. T. H., Nguyen, T. T. L., & Dinh, T. S. (2017). Genetic diversity among 25 accessions of Camellia spp. collected in Quang Ninh by RAPD and ISSR markers. Vietnam Agricultural Science Journal, 15(8), 1077-1092.
Gao, J., Parks, C. R., & Du, Y. (2005). Collected species of the genus Camellia – An illustrated outline. Zhejiang Science and Technology Press.
Hamrick, J. L., & Godt, M. J. W. (1996). Allozyme diversity in cultivated crops. Crop Science, 37(1), 26-30. https://doi.org/10.2135/cropsci1997.0011183X003700010004x
Hogbin, P. M., & Peakall, R. (1999). Evaluation of the contribution of genetic research to the management of the endangered plant Zieria prostrata. Conservation Biology, 13(3), 514-522. http://www.jstor.org/stable/2641866
Kumar, P., Gupta, V. K., Misra, A. K., Modi, D. R., & Pandey, B. K. (2009). Potential of molecular markers in plant biotechnology. Plant Omics, 2(4), 141-162. https://www.researchgate.net/publication/222177409_Potential_of_Molecular_Markers_in_Plant_Biotechnology
Luu, T. T., La, A. D., Tran, V. T., Le, N. T., & Phi, H. H. (2020). Genetic diversity of naturally distributed Rhododendron moulmainense Hook. f. populations in Lam Vien plateau, Vietnam revealed by ISSR and SCoT markers. Malaysian Applied Biology, 49(5), 41-52. http://journalarticle.ukm.my/17383/1/49_05_05.pdf
Nagaoka, T., & Ogihara, Y. (1997). Applicability of inter-simple sequence repeat polymorphism in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and Applied Genetics, 94, 597-602. https://doi.org/10.1007/s001220050456
Nybom, H., & Bartish, I. V. (2000). Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspectives in Plant, Ecology, Evolution and Systematics, 3(2), 93-114. https://doi.org/10.1078/1433-8319-00006
Ouborg, N. J., Piquot, Y., & Van Groenendael, J. M. (1999). Population genetics, molecular markers and the study of dispersal in plants. Journal of Ecology, 87(4), 551-568. https://doi.org/10.1046/j.1365-2745.1999.00389.x
Rohlf, F. J. (2004). NTSYSpc numerical taxonomy and multivariate analysis system version 2.1 – User guide. Applied Biostatistics Inc.
Tang, S., Bin, X., Wang, L., & Zhong, Y. (2006). Genetic diversity and population structure of yellow camellia (Camellia nitidissima) in China as revealed by RAPD and AFLP markers. Biochemical Genetics, 44(9-10), 449-461. https://doi.org/10.1007/s10528-006-9053-y
Tran, D. M., Nguyen, T. T., Hoang, T. S., Dang, V. T., Phung, D. T., Nguyen, V. T., Dao, T. D., Mai, T. L., Vu, T. L., Nguyen, H. T., Nguyen, T. T. P., & Tran, V. D. (2019). Golden camellias: A review. Archives of Current Research International, 16(2), 1-8. https://doi.org/10.9734/acri/2019/v16i230085
Tran, N., & Luong, V. D. (2013). Camellia dilinhensis – A new yellow species from Vietnam. International Camellia Journal, 45, 87-89.
Vijayan, K., Zhang, W., & Tsou, C. (2009). Molecular taxonomy of Camellia (Theaceae) inferred from nrITS sequences. American Journal of Botany, 96(7), 1348-1360. https://doi.org/10.3732/ajb.0800205
Wang, B. Y., & Ruan, Z. Y. (2012). Genetic diversity and differentiation in Camellia reticulata (Theaceae) polyploid complex revealed by ISSR and ploidy. Genetics and Molecular Research, 11(1), 503-511. https://doi.org/10.4238/2012.March.6.3
Wei, X., Cao, H. -L., Jiang, Y. -S., Ye, W. -H., Ge, X. -J., & Li, F. (2008). Population genetic structure of Camellia nitidissima (Theaceae) and conservation implications. Botanical Studies, 49(2), 147-153. https://ejournal.sinica.edu.tw/bbas/content/2008/2/Bot492-07.pdf
Wei, X., Wei, J.-Q., Cao, H.-L., Li, F., & Ye, W.-H. (2005). Genetic diversity and differentiation of Camellia euphlebia (Theaceae) in Guangxi, China. Annales Botanici Fennici, 42(5), 365-370. http://www.jstor.org/stable/23726731
Weising, K., Nybom, H., Wolff, K., & Kahl, G. (2005). DNA fingerprinting in plants: Principles, methods, and applications (2nd ed.). CRC Press, Taylor & Francis Group. https://doi.org/10.1201/9781420040043
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22), 6531-6535. https://doi.org/10.1093/nar/18.22.6531
Yeh, F. C., Yang, R. C., & Boyle, T. (1999). POPGENE version 1.32: Microsoft Windows-based freeware for population genetics analysis. University of Alberta, Edmonton.
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